Starch Content in the Process Water (enzymatic hydrolysis)
The starch content of process water samples is determined using the RH 24/09 method developed by PTS. The method is used for the quantitative analytical determination of starch in process waters with a consistency < 1%. The process water samples are adjusted to the pH range effective for the enzyme amyloglucosidase by means of diluted acetic acid and acetate buffer. Addition of the enzyme and tempering results in hydrolysis of the starch to glucose.
The glucose content is determined quantitatively by means of a biosensor and converted into the starch content. The starch content of process water samples should be determined immediately after sampling or in the preserved state. The starch content is expressed in [mg starch/ L] or in [%]. The detection limit of this method is 400 mg/L.